Szabo, GyongyiMandrekar, Pranoti2022-08-232022-08-232008-03-292010-04-21Methods Mol Biol. 2008;447:113-24. <a href="http://dx.doi.org/10.1007/978-1-59745-242-7_9">Link to article on publisher's site</a>1064-3745 (Linking)10.1007/978-1-59745-242-7_918369915https://hdl.handle.net/20.500.14038/31151Both acute and chronic alcohol consumption have significant immunomodulatory effects of which alterations in innate immune functions contribute to impaired antimicrobial defense and inflammatory responses. Blood monocytes, macrophages, and dendritic cells play a central role in innate immune recognition as these cells recognize pathogens, respond with inflammatory cytokine production, and induce antigen-specific T-lymphocyte activation. All of these innate immune cell functions are affected in humans by alcohol intake. Here, we summarize the different effects of acute and chronic alcohol on monocyte, macrophage, and dendritic cell functions in humans and describe methods for separation and functional evaluation of these cell types.en-US*Cell Culture TechniquesCell SeparationCells, CulturedCytokinesDendritic CellsDose-Response Relationship, DrugElectrophoretic Mobility Shift AssayEthanolHumansImmunity, InnateLymphocyte Culture Test, MixedMacrophagesMonocytesNF-kappa BBiochemistry, Biophysics, and Structural BiologyGastroenterologyImmunology and Infectious DiseaseMolecular BiologyJournal Articlehttps://escholarship.umassmed.edu/gastroenterology_pp/751282375gastroenterology_pp/75