Shaw, Leslie M.Mercurio, Arthur M.2022-08-232022-08-231989-01-012010-11-07J Exp Med. 1989 Jan 1;169(1):303-8. <a href="http://dx.doi.org/10.1084/jem.169.1.303">Link to article on publisher's website</a>0022-1007 (Linking)10.1084/jem.169.1.3032491881https://hdl.handle.net/20.500.14038/26204The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adherence, however, is observed when IFN-gamma (greater than or equal to 5 U/ml) is used together with LPS (2.0 ng/ml). These requirements parallel the conditions needed to obtain tumoricidal activation of TG-elicited macrophages. Adherence to laminin, in the presence of these stimuli, is transient, being maximal at 8 h after their addition and diminishing with longer periods of incubation. In contrast, adherence to type IV collagen does not appear to be transient and IFN-gamma and LPS induce a more prolonged association of macrophages with this substratum.en-USAnimalsBasement MembraneCell AdhesionCollagenFibronectinsInterferon-gammaLamininLipopolysaccharidesMacrophage ActivationMacrophagesMiceThioglycolatesTime FactorsCancer BiologyNeoplasmsJournal Articlehttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1113&amp;context=cancerbiology_pp&amp;unstamped=1https://escholarship.umassmed.edu/cancerbiology_pp/1131633338cancerbiology_pp/113