Connie CepkoChinchore, YashodhanBegaj, TediWu, DavidDrokhlyansky, EugeneCepko, Constance L.2022-08-232022-08-232017-06-092018-05-10<p>Elife. 2017 Jun 9;6. doi: 10.7554/eLife.25946. <a href="https://doi.org/10.7554/eLife.25946">Link to article on publisher's site</a></p>2050-084X (Linking)10.7554/eLife.2594628598329https://hdl.handle.net/20.500.14038/49323<p>Tedi Begaj participated in this study as a medical student as part of the Senior Scholars research program at the University of Massachusetts Medical School. </p>Vertebrate photoreceptors are among the most metabolically active cells, exhibiting a high rate of ATP consumption. This is coupled with a high anabolic demand, necessitated by the diurnal turnover of a specialized membrane-rich organelle, the outer segment, which is the primary site of phototransduction. How photoreceptors balance their catabolic and anabolic demands is poorly understood. Here, we show that rod photoreceptors in mice rely on glycolysis for their outer segment biogenesis. Genetic perturbations targeting allostery or key regulatory nodes in the glycolytic pathway impacted the size of the outer segments. Fibroblast growth factor signaling was found to regulate glycolysis, with antagonism of this pathway resulting in anabolic deficits. These data demonstrate the cell autonomous role of the glycolytic pathway in outer segment maintenance and provide evidence that aerobic glycolysis is part of a metabolic program that supports the biosynthetic needs of a normal neuronal cell type.en-USCopyright Chinchore et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.http://creativecommons.org/licenses/by/4.0/Warburg effectallosterycell biologymouseneuroscienceouter segmentsretinal metabolismBiochemical Phenomena, Metabolism, and NutritionCell BiologyCellular and Molecular PhysiologyMedical EducationNeuroscience and NeurobiologyGlycolytic reliance promotes anabolism in photoreceptorsJournal Articlehttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1265&context=ssp&unstamped=1https://escholarship.umassmed.edu/ssp/26412106905ssp/264