Zhang, YingXie, RonglinGordon, Jonathan A. R.LeBlanc, Kimberly T.Stein, Janet L.Lian, Jane B.Van Wijnen, Andre J.Stein, Gary S.2022-08-232022-08-232012-04-272012-05-07J Biol Chem. 2012 Apr 27. [Epub ahead of print] doi:10.1074/jbc.M112.3403981083-351X10.1074/jbc.M112.34039822544738https://hdl.handle.net/20.500.14038/49587Multiple microRNAs (miRNAs) that target the osteogenic Runt-related transcription factor 2 (RUNX2) define an interrelated network of miRNAs that control osteoblastogenesis. We addressed whether these miRNAs have functional targets beyond RUNX2 that co-regulate skeletal development. Here, we find that seven RUNX2-targeting miRNAs (miR-23a, miR-30c, miR-34c, miR-133a, miR-135a, miR-205 and miR-217) also regulate the chondrogenic GATA transcription factor Tricho-Rhino-Phalangeal Syndrome I (TRPS1). While the efficacy of each miRNA to target RUNX2 or TRPS1 differs in osteoblasts and chondrocytes, each effectively blocks maturation of pre-committed osteoblasts and chondrocytes. Furthermore, these miRNAs can redirect mesenchymal stem cells into adipogenic cell fate with concomitant up-regulation of key lineage specific transcription factors. Thus, a program of multiple miRNAs controls mesenchymal lineage progression by selectively blocking differentiation of osteoblasts and chondrocytes to control skeletal development.en-USMicroRNAsOsteoblastsChondrocytesDNA-Binding ProteinsTranscription FactorsCore Binding Factor Alpha 1 SubunitMesenchymal Stem CellsMusculoskeletal DevelopmentCell BiologyJournal Articlehttps://escholarship.umassmed.edu/stein/2572826549stein/257