Gerami, PedramCook, Robert W.Wilkinson, JeffRussell, Maria C.Dhillon, NavneetAmaria, Rodabe N.Gonzalez, ReneLyle, StephenJohnson, Clare E.Oelschlager, Kristen M.Jackson, Gilchrist L.Greisinger, Anthony J.Maetzold, DerekDelman, Keith A.Lawson, David H.Stone, John F.2022-08-232022-08-232015-01-012015-04-24Clin Cancer Res. 2015 Jan 1;21(1):175-83. doi: 10.1158/1078-0432.CCR-13-3316. <a href="http://dx.doi.org/10.1158/1078-0432.CCR-13-3316">Link to article on publisher's site</a>1078-0432 (Linking)10.1158/1078-0432.CCR-13-331625564571https://hdl.handle.net/20.500.14038/36525PURPOSE: The development of a genetic signature for the identification of high-risk cutaneous melanoma tumors would provide a valuable prognostic tool with value for stage I and II patients who represent a remarkably heterogeneous group with a 3% to 55% chance of disease progression and death 5 years from diagnosis. EXPERIMENTAL DESIGN: A prognostic 28-gene signature was identified by analysis of microarray expression data. Primary cutaneous melanoma tumor tissue was evaluated by RT-PCR for expression of the signature, and radial basis machine (RBM) modeling was performed to predict risk of metastasis. RESULTS: RBM analysis of cutaneous melanoma tumor gene expression reports low risk (class 1) or high risk (class 2) of metastasis. Metastatic risk was predicted with high accuracy in development (ROC = 0.93) and validation (ROC = 0.91) cohorts of primary cutaneous melanoma tumor tissue. Kaplan-Meier analysis indicated that the 5-year disease-free survival (DFS) rates in the development set were 100% and 38% for predicted classes 1 and 2 cases, respectively (P < 0.0001). DFS rates for the validation set were 97% and 31% for predicted classes 1 and 2 cases, respectively (P < 0.0001). Gene expression profile (GEP), American Joint Committee on Cancer stage, Breslow thickness, ulceration, and age were independent predictors of metastatic risk according to Cox regression analysis. CONCLUSIONS: The GEP signature accurately predicts metastasis risk in a multicenter cohort of primary cutaneous melanoma tumors. Preliminary Cox regression analysis indicates that the signature is an independent predictor of metastasis risk in the cohort presented.en-USCancer BiologyCell BiologyGeneticsMolecular BiologyNeoplasmsJournal Articlehttps://escholarship.umassmed.edu/mccb_pubs/197027665mccb_pubs/19