Koupenova-Zamor, MilkaMick, Eric O.Corkrey, Heather A.Singh, AnupamaTanriverdi, Selim E.Vitseva, OlgaLevy, DanielKeeler, Allison MEzzaty Mirhashemi, MarziehElmallah, Mai K.Gerstein, MarkRozowsky, JoelTanriverdi, KahramanFreedman, Jane E.2022-08-232022-08-232019-09-272019-10-17<p>iScience. 2019 Sep 27;19:916-926. doi: 10.1016/j.isci.2019.08.035. Epub 2019 Aug 24. <a href="https://doi.org/10.1016/j.isci.2019.08.035">Link to article on publisher's site</a></p>2589-0042 (Linking)10.1016/j.isci.2019.08.03531518900https://hdl.handle.net/20.500.14038/41196The presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence of these pine-pollen miRNAs associated with hay fever and not with overt cardiovascular or pulmonary disease. Using in vivo and in vitro models, we found that transmission of pollen-miRNAs into the circulation occurs via pulmonary transfer and this transfer was mediated by platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake. These data demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans. Although these data suggest mechanistic plausibility for pulmonary-mediated plant-derived miRNA transfer into the human circulation, our large observational cohort data do not implicate major disease or risk factor association.en-USCopyright 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).http://creativecommons.org/licenses/by-nc-nd/4.0/Biological SciencesMolecular BiologyOmicsTranscriptomicsCardiovascular DiseasesGenetics and GenomicsMolecular BiologyNucleic Acids, Nucleotides, and NucleosidesPopulation BiologyRespiratory SystemRespiratory Tract DiseasesJournal Articlehttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5000&amp;context=oapubs&amp;unstamped=1https://escholarship.umassmed.edu/oapubs/398315570734oapubs/3983