Kurane, IchiroWest, KimTuazon, Carmelita U.Zeng, WeilanEnnis, Francis A.2022-08-232022-08-232003-05-012017-08-22J Clin Virol. 2003 May;27(1):38-43. doi: 10.1016/S1386-6532(02)00109-91386-6532 (Linking)10.1016/S1386-6532(02)00109-912727527https://hdl.handle.net/20.500.14038/35060We have established 3 CD8+ cytotoxic T lymphocyte (CTL) clones from the peripheral blood mononuclear cells of two HIV-1-seropositive asymptomatic donors. The epitopes recognized by these CTL clones were defined using synthetic peptides. The epitopes were located on HIV-1gag protein between amino acid (a.a.) 145 and 155 (QAISPRTLNAW), a.a.193 and 201 (GHQAAMQML), and a.a.260 and 267 (EIYKRWII), and were presented by HLA-A25, HLA-B38 and HLA-B8, respectively. The former 2 epitopes have not been previously defined. The HLA-A25-restricted epitope overlapped with HLA-B57-restricted and HLA-Cw3-restricted epitopes previously reported. In addition, this epitope overlapped with an HLA-DQ-restricted epitope recognized by CD4+ CTL. The HLA-B38-restricted epitope overlapped with HLA-A2-restricted and HLA-Bw52-restricted epitopes that were previously reported. The HLA-B38-restricted epitope between a.a.193 and 201 was highly conserved among HIV-1 strains. The results demonstrate that two new epitopes were defined in a region of gag protein that includes multiple epitopes presented by multiple HLA.en-USImmunityImmunology and Infectious DiseaseImmunology of Infectious DiseaseInfectious DiseaseVirologyJournal Articlehttps://escholarship.umassmed.edu/infdis_pp/27310636649infdis_pp/273