Chen, ZexiangKwan, Suet-YanMir, AamirHazeltine, MaxShin, MinwookLiang, Shun-QingChan, Io LongKelly, KarenGhanta, Krishna SGaston, NicholasCao, YueyingXie, JunGao, GuangpingXue, WenSontheimer, Erik JWatts, Jonathan K2024-02-232024-02-232023-12-13Chen Z, Kwan SY, Mir A, Hazeltine M, Shin M, Liang SQ, Chan IL, Kelly K, Ghanta KS, Gaston N, Cao Y, Xie J, Gao G, Xue W, Sontheimer EJ, Watts JK. A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors. CRISPR J. 2023 Dec;6(6):570-582. doi: 10.1089/crispr.2023.0048. PMID: 38108517; PMCID: PMC10753986.2573-160210.1089/crispr.2023.004838108517https://hdl.handle.net/20.500.14038/53086CRISPR-based genome-editing technologies, including nuclease editing, base editing, and prime editing, have recently revolutionized the development of therapeutics targeting disease-causing mutations. To advance the assessment and development of genome editing tools, a robust mouse model is valuable, particularly for evaluating in vivo activity and delivery strategies. In this study, we successfully generated a knock-in mouse line carrying the Traffic Light Reporter design known as TLR-multi-Cas variant 1 (TLR-MCV1). We comprehensively validated the functionality of this mouse model for both in vitro and in vivo nuclease and prime editing. The TLR-MCV1 reporter mouse represents a versatile and powerful tool for expediting the development of editing technologies and their therapeutic applications.enJournal ArticleThe CRISPR journal