Murray, LiamOlson, Meagan NBarton, NathanielDawes, PepperChan, YingleongLim, Elaine T2023-08-152023-08-152023-06-11Murray L, Olson MN, Barton N, Dawes P, Chan Y, Lim ET. FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids. Methods Mol Biol. 2023;2683:193-199. doi: 10.1007/978-1-0716-3287-1_15. PMID: 37300776.1940-602910.1007/978-1-0716-3287-1_1537300776https://hdl.handle.net/20.500.14038/52418Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cerebral organoids to identify or validate genotype-to-cell type associations. Our approach is high-throughput and quantitative and uses internal controls to enable comparisons of the results across different antibody markers and experiments.en© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Cerebral organoidsFACSGenotype to cell typeSequencingoFlowSeqBook ChapterMethods in molecular biology (Clifton, N.J.)