Nwosu, Benjamin U.Soyka, Leslie A.Angelescu, AmandaLee, Mary M.2022-08-232022-08-232011-04-142011-09-06J Pediatr Endocrinol Metab. 2011;24(3-4):163-7. <a href="http://dx.doi.org/10.1515/JPEM.2011.088">Link to article on publisher's website</a>0334-018X (Linking)10.1515/JPEM.2011.08821648284https://hdl.handle.net/20.500.14038/43757OBJECTIVES: The ternary complex is composed of insulin-like growth factor (IGF)-I, IGF binding protein (IGFBP)-3 and acid labile subunit (ALS). Growth hormone (GH) promotes IGFBP-3 proteolysis to release free IGF-I, ALS, and IGFBP-3 fragments. Our aim was to determine whether elevated GH levels during GH stimulation testing would trigger IGFBP-3 proteolysis. DESIGN: This prospective study of 10 short prepubertal children (height standard deviation score -2.37 +/- 0.31) used arginine and GH releasing hormone stimulation to study dynamic changes in the ternary complex moieties. IGFBP-3 was measured in two assays: a radioimmunoassay (RIA) that detects both cleaved and intact IGFBP-3; and an immunochemiluminescence assay (ICMA) that detects only intact IGFBP-3. RESULTS: IGFBP-3 measured by RIA increased by 19% (p < 0.05), while IGFBP-3 measured by ICMA did not significantly increase (6.1%). CONCLUSION: The significant increase in IGFBP-3 measured by RIA, but not ICMA, provides evidence of IGFBP-3 proteolysis during acute GH stimulation.en-USArginineChildDwarfismFemaleGrowth Hormone-Releasing HormoneHuman Growth HormoneHumansInsulin-Like Growth Factor Binding Protein 3MaleProspective StudiesPubertyEndocrinology, Diabetes, and MetabolismPediatricsJournal Articlehttps://escholarship.umassmed.edu/peds_pp/72221121peds_pp/7