Roy, Kanaklatade la Serna, Ivana L.Imbalzano, Anthony N.2022-08-232022-08-232002-07-102008-08-04J Biol Chem. 2002 Sep 13;277(37):33818-24. Epub 2002 Jul 8. <a href="http://dx.doi.org/10.1074/jbc.M205159200">Link to article on publisher's site</a>0021-9258 (Print)10.1074/jbc.M20515920012105204https://hdl.handle.net/20.500.14038/42373The myogenic basic helix-loop-helix family of transcription factors, MyoD, Myf5, myogenin, and MRF4, can each activate the muscle differentiation program when ectopically expressed in non-muscle cells. SWI/SNF complexes are ATP-dependent chromatin remodeling enzymes. We demonstrated previously that SWI/SNF enzymes promote MyoD-mediated muscle differentiation. To ascertain the requirement for SWI/SNF enzymes in muscle differentiation mediated by different MyoD family members, we examined MyoD, Myf5, MRF4, and myogenin-mediated induction of muscle differentiation in cells expressing dominant negative versions of BRG1 or BRM-based SWI/SNF enzymes. We demonstrated that expression of dominant negative BRG1 or BRM inhibited the induction of muscle-specific gene expression by Myf5 and MRF4; however, myogenin failed to induce measurable quantities of muscle-specific mRNAs, even in cells not expressing dominant negative SWI/SNF. In contrast, all four myogenic regulators induced expression of the cell cycle regulators p21, Rb, and cyclin D3 and promoted cell cycle arrest independently of the SWI/SNF enzymes. We proposed that SWI/SNF enzymes are required for the induction of all muscle-specific gene expression by MyoD, Myf5, and MRF4, whereas induction of the cell cycle regulators, p21, Rb, and cyclin D3 occurred independently of SWI/SNF function.en-US3T3 CellsAnimalsCell CycleCell DifferentiationChromatinChromosomal Proteins, Non-HistoneCyclins*DNA-Binding ProteinsGene Expression Regulation, DevelopmentalHelix-Loop-Helix MotifsHumansMiceMuscle ProteinsMuscle, SkeletalMyoD ProteinMyogenic Regulatory Factor 5Myogenic Regulatory FactorsMyogenin*Trans-ActivatorsTranscription FactorsCell BiologyLife SciencesMedicine and Health SciencesJournal Articlehttps://escholarship.umassmed.edu/oapubs/728564643oapubs/728