Hehnly, HeidiDoxsey, Stephen J.2022-08-232022-08-232014-03-102014-04-14Hehnly H, Doxsey S. Rab11 endosomes contribute to mitotic spindle organization and orientation. Dev Cell. 2014 Mar 10;28(5):497-507. doi:10.1016/j.devcel.2014.01.014. <a href="http://dx.doi.org/10.1016/j.devcel.2014.01.014">Link to article on publisher's site</a>1534-5807 (Linking)10.1016/j.devcel.2014.01.01424561039https://hdl.handle.net/20.500.14038/44475During interphase, Rab11-GTPase-containing endosomes recycle endocytic cargo. However, little is known about Rab11 endosomes in mitosis. Here, we show that Rab11 localizes to the mitotic spindle and regulates dynein-dependent endosome localization at poles. We found that mitotic recycling endosomes bind gamma-TuRC components and associate with tubulin in vitro. Rab11 depletion or dominant-negative Rab11 expression disrupts astral microtubules, delays mitosis, and redistributes spindle pole proteins. Reciprocally, constitutively active Rab11 increases astral microtubules, restores gamma-tubulin spindle pole localization, and generates robust spindles. This suggests a role for Rab11 activity in spindle pole maturation during mitosis. Rab11 depletion causes misorientation of the mitotic spindle and the plane of cell division. These findings suggest a molecular mechanism for the organization of astral microtubules and the mitotic spindle through Rab11-dependent control of spindle pole assembly and function. We propose that Rab11 and its associated endosomes cocontribute to these processes through retrograde transport to poles by dynein.en-USBiochemistryCell BiologyDevelopmental BiologyJournal Articlehttps://escholarship.umassmed.edu/pmm_pp/75476837pmm_pp/7