Murphy, Kenan C.2022-08-232022-08-231998-04-292008-08-04J Bacteriol. 1998 Apr;180(8):2063-71.0021-9193 (Print)9555887https://hdl.handle.net/20.500.14038/42298Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.en-USBacteriophage lambdaCalciumChromosomes, BacterialCloning, MolecularDNA PrimersDNA, SuperhelicalEscherichia coliGene TargetingGenotypeOperonPlasmidsPolymerase Chain ReactionRecombinant Fusion Proteins*Recombination, GeneticRestriction MappingLife SciencesMedicine and Health SciencesJournal Articlehttps://escholarship.umassmed.edu/oapubs/660564468oapubs/660