Target accessibility dictates the potency of human RISC
| dc.contributor.author | Brown, Kirk M. | |
| dc.contributor.author | Chu, Chia-ying | |
| dc.contributor.author | Rana, Tariq M. | |
| dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
| dc.contributor.department | Morningside Graduate School of Biomedical Sciences | |
| dc.date | 2022-08-11T08:08:50.000 | |
| dc.date.accessioned | 2022-08-23T16:09:46Z | |
| dc.date.available | 2022-08-23T16:09:46Z | |
| dc.date.issued | 2005-04-27 | |
| dc.date.submitted | 2008-08-11 | |
| dc.description.abstract | In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis. | |
| dc.identifier.citation | Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. <a href="http://dx.doi.org/10.1038/nsmb931">Link to article on publisher's site</a> | |
| dc.identifier.contextkey | 573959 | |
| dc.identifier.doi | 10.1038/nsmb931 | |
| dc.identifier.issn | 1545-9993 (Print) | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/133 | |
| dc.identifier.pmid | 15852021 | |
| dc.identifier.submissionpath | gsbs_sp/133 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14038/32776 | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15852021&dopt=Abstract ">Link to article in PubMed</a> | |
| dc.relation.url | http://dx.doi.org/10.1038/nsmb931 | |
| dc.source.issue | 5 | |
| dc.source.journaltitle | Nature structural and molecular biology | |
| dc.source.pages | 469-70 | |
| dc.source.volume | 12 | |
| dc.title | Target accessibility dictates the potency of human RISC | |
| dc.type | Journal Article | |
| dspace.entity.type | Publication | |
| html.description.abstract | <p>In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.</p> |